Uppsala universitet

Welcome to our Chlamydia trachomatis multilocus sequence typing (MLST) database!

This high resolution genotyping system has been developed for epidemiological studies of C. trachomatis infections. It is based on five target regions and in addition contains information about the commonly used ompA gene. Our MLST system has a threefold higher resolution than ordinary ompA genotyping [ Klint et al., 2007].

The system has been applied to >2000 specimens collected from a variety of countries and patient populations including the three different types of C. trachomatis infections: Urogenital chlamydia, lymphogranuloma venereum (LGV) and trachoma. See reference list below. The original MLST system published by Klint et al. in 2007 have since then been modified by shortening some of the target regions and by introducing nested PCRs [ Bom et al. 2011].
A protocol for the currently used PCR system can be found here.

Available databases

Current database (based on the modified MLST system by Bom et al.) Contains >400 STs obtained from >2000 specimens.
Old database (based on the original MLST system by Klint et al.) Contains 221 STs obtained from 971 specimens.

If you have data generated with the original MLST system and wish to compare it with data generated with the modified MLST system you can use the following script (excel-file) to convert the data: Download Conversion script.

The MLST system has also been adapted to a microarray format [ Christerson et al., 2011].

If you are interested in more background information about the specimens or have other questions, please contact Björn Herrmann.

Emergence and Spread of Chlamydia trachomatis Variant, Sweden

Reference list of articles where the MLST system has been applied:

  1. Klint M, Fuxelius HH, Goldkuhl RR, Skarin H, Rutemark C, Andersson SG, Persson K, Herrmann B. High-resolution genotyping of Chlamydia trachomatis strains by multilocus sequence analysis. J Clin Microbiol. 2007;45:1410-4.
  2. Herrmann B, Törner A, Low N, Klint M, Nilsson A, Velicko I, Söderblom T, Blaxhult A. Emergence and spread of Chlamydia trachomatis variant, Sweden. Emerg Infect Dis 2008;14:1462-5.
  3. Jurstrand M, Christerson L, Klint M, Fredlund M, Unemo M, Herrmann B. Characterization of Chlamydia trachomatis by ompA sequencing and multilocus sequence typing (MLST) in a Swedish county before and after identification of the new variant. Sex Transm Inf, 2010;86:56-60. Erratum in: Sex Transm Infect. 2010;86:250.
  4. Klint M, Thollesson M, Bongcam-Rudloff, Birkelund S, Nilsson A, Herrmann B. Mosaic structure of intragenic repetitive elements in histone H1-like protein Hc2 varies within serovars of Chlamydia trachomatis. BMC Microbiology 2010 Mar 17;10:81.
  5. Harding-Esch et al. Multi-locus sequence typing: a useful tool for trachoma molecular epidemiology. In: Stary, Byrne, Caldwell et al., editors. Chlamydial infections. Proceedings of the 12th International Symposium on Human Chlamydial Infections; Salzburg 2010, p. 55-58.]
  6. Klint M, Hadad R, Christerson L, Loré B, Anagrius C, Österlund A, Larsson I, Sylvan S, Fredlund H, Unemo M, Herrmann B. Prevalence trends in Sweden for the new variant of Chlamydia trachomatis. Clin Microbiol Infect. 2011;17:683-9
  7. Christerson L, de Vries H, Barbeyrac B, Gaydos CA, Henrich B, Hoffmann S, Schachter J, Thorvaldsen J, Vall-Mayans M, Klint M et al: Typing of Lymphogranuloma Venereum Chlamydia trachomatis Strains. Emerg Infect Dis. 2010;16:1777-9.
  8. Christerson L, de Vries H, Klint M, Herrmann B, Morré SA. Multilocus sequence typing of urogenital Chlamydia trachomatis from patients with different degrees of clinical symptoms. Sex Transm Dis 2011;38:490-494.
  9. Bom RJ, Christerson L, Schim van der Loeff MF, Coutinho RA, Herrmann B, Bruisten SM. Evaluation of high resolution typing methods for Chlamydia trachomatis in heterosexual couples. J Clin Microbiol 2011;49:2844–2853.
  10. Gravningen K, Christerson L, Furberg AS, Simonsen GS, Odman K, Ståhlsten A, Herrmann B. Multilocus Sequence Typing of Genital Chlamydia trachomatis in Norway Reveals Multiple New Sequence Types and a Large Genetic Diversity. PLoS One. 2012;7(3):e34452.
  11. Labiran C, Clarke IN, Cutcliffe LT, Wang Y, Skilton RJ, Persson K, Bjartling, C, Herrmann B, Christerson L, Marsh P. Genotyping markers used for multi locus VNTR analysis with ompA (MLVA-ompA) and multi sequence typing (MST) retain stability in Chlamydia trachomatis. Front Cell Infect Microbiol. 2012;2:68.
  12. Christerson L, Bom RJ, Bruisten SM, Yass R, Hardick J, Bratt G, Gaydos CA, Morré SA, Herrmann B. Chlamydia trachomatis strains show specific clustering for men who have sex with men compared to heterosexual transmission in Sweden, the Netherlands and the United States. J Clin Microbiol. 2012;50:3548-55.
  13. Bom RJ, van der Helm JJ, Schim van der Loeff MF, van Rooijen MS, Heijman T, Matser A, de Vries HJ, Bruisten SM. Distinct transmission networks of Chlamydia trachomatis in men who have sex with men and heterosexual adults in Amsterdam, The Netherlands. PLoS One. 2013;8(1):e53869. doi: 10.1371/journal.pone.0053869.
  14. Bom RJ, Matser A, Bruisten SM, van Rooijen MS, Heijman T, Morré SA, de Vries HJ, van der Loeff MF. Multilocus sequence typing of Chlamydia trachomatis among men who have sex with men reveals co-circulating strains not associated with specific subpopulations. J Infect Dis. 2013 Jun 17. [Epub ahead of print] PubMed PMID: 23776193.]

This Multilocus Sequence Typing (MLST) database is engineered/hosted by the Computing Department at BMC, Uppsala Biomedical Centre, Uppsala University.